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Srdx repressor1/6/2024 ![]() The mutant WUS protein, WUSm1, which carries a mutated version of the WUS-box, appeared to have a reduced ability to induce adventitious organs. The WUS-box might also be important for the ability of Arabidopsis WUS to induce adventitious organogenesis. We have shown that the WUS-box is a bifunctional domain, behaving as an activator domain for the induction of AGAMOUS ( AG) expression and acting as a repressor domain for the suppression of the expression of type-A ARABIDOPSIS RESPONSE REGULATOR ( ARR) genes. Although both domains show strong repressive activity in transient expression assays, the biological functions of each domain are different. We have previously discovered and reported that WUS has two functional domains: the WUS-box and the ethylene-responsive element binding factor-associated amphiphilic repression (EAR)-like motif. We reasoned that a detailed analysis of Arabidopsis WUS and LEC1 molecular functions might yield some clues that would allow us to enhance their ability to induce adventitious organogenesis. Our results show that the modification of transcriptional repression/activation activity offers an effective method to improve the efficiency of adventitious organ formation in plants. Upon overexpression of the corresponding transgenes, we succeeded in improving the efficiency of adventitious organ induction. Conversely, we fused the strong transcriptional activation domain VP16 from herpes simplex virus to LEC1. Because WUS functions as a transcriptional repressor during the induction of adventitious organs, we fused it to an artificial strong repression domain, SUPERMAN REPRESSION DOMAIN X (SRDX). Here, we show that modifying the transcriptional repression/activation activities of WUS and LEC1 improves the efficiency of adventitious organ formation in Arabidopsis. Even though the Arabidopsis ( Arabidopsis thaliana) WUSCHEL ( WUS) and LEAFY COTYLEDON 1 ( LEC1) genes can induce adventitious organ formation in Arabidopsis without phytohormone treatment, further improvement is desired. In recent years, several genes have been reported to be adventitious organ inducers and proposed to be useful for industrial applications. The induction of adventitious organs, such as calli, shoots, and somatic embryos, in tissue culture is a useful technique for plant propagation and genetic modification.
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